Pericles Assad Hassun Filho
Pericles Assad Hassun Filho
Dr Hassun Filho graduated in the following: Veterinary Medicine at the Universidade Estadual Paulista Julio de Mesquita Filho (1990), Master of Animal Reproduction, University of São Paulo (1999), Doctor in Clinical Endocrinology (2001) and postdoctoral in Structural Molecular Biology (2002) at Federal University of São Paulo. He is currently CEO of Genesis Genetics Brasil (InGene Biotechnology Ltd.) and a member of the Managing Committee of the National Institute of Science and Technology of “Stem Cells in Human Genetic Diseases” associated with the Center for Human Genome Studies, University of São Paulo. Dr Hassun Filho is working in the following areas: biotechnology as applied to reproduction, genetics of human infertility and pre-implantation genetic diagnosis.
Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary for future application of hESCs in cell theapy. However, the majority of hESC lines worldwide have been established and serially propagated on inactivated murine embryonic fibroblast (MEF) feeders in the presence of culture medium containing animal-based components, thereby severely limiting their potential for human clinical application. The development and refinement of xeno-free culture systems, that avoid the risk of hESC contamination with adventitious agents, and support undifferentiated hESC populations capable of the massive scale-up of hESC numbers necessary for long term storage is essential.My main contribution in CETGEN will be to introduce/improve protocols to: avoid xeno-contamination during derivation and culture of hESCs, to derive and propagate fibroblast human feeder layers in a xeno-free medium supplemented with recombinant human serum and replaces immunosurgery, involving the use of guinea pig complement, with a modified mechanical derivation procedure. In addition, to isolate new human feeder cell lines for hESC derivation under good manufacturing practice conditions, and involving the screening for human and animal pathogens to ensure the feeder cell suitability for the derivation of therapy-grade hESCs. Another attainable goal would be use my experience in Preimplantation Genetic Diagnosis (PGD) to establish new hESC lines carrying a clinically relevant monogenic or chromosomal defect to study the pathophysiology of these diseases. In particular, such stem cell lines provide unique opportunities to see how chromosome aneuploidies impact stem cell in vitro growth and differentiation potential.
Diagnostic and counselling activitivities
Dr Hassun Filho is specialized in Preimplantation Genetic Diagnosis and Assisted Reproductive Technology counselling”. Although this is not currently an ongoing CEPID activity, it is anticipated that gradually more requests for diagnosis and counseling in assisted reproductive technologies will emerge. Particularly in Sao Paulo, where the majority of the Brazilian ART centers are located, patients known to be carriers of genetic disorders may seek PGD to prevent disease transmission to subsequent generations without the repugnancy of pregnancy termination.
Favorite 5 publications
- Bertolla, R.P., Cedenho, A.P., Hassun Filho, P.A., Lima, S.B., Ortiz, V. and Srougi, M. (2006) Sperm nuclear DNA fragmentation in adolescents with varicocele. Fertil Steril, 85, 625-628.
- Hassun Filho, P.A., Ribeiro, M.A., da Rocha, A.M., Allegreti, J.R., Serafini, P.C. and da Motta, E.L. (2008) Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting. J Assist Reprod Genet, 25, 467-471.
- Lima, S.B., Cenedeze, M.A., Bertolla, R.P., Filho, P.A., Oehninger, S. and Cedenho, A.P. (2006) Expression of the HSPA2 gene in ejaculated spermatozoa from adolescents with and without varicocele. Fertil Steril, 86, 1659-1663.
- Serafini, P., Da Rocha, A.M., De Toledo Osorio, C.A., Smith, G.D., Hassun, P.A., da Silva, I.G., Da Motta, E.L. and Baracat, E.C. (2009) Protein profile of the luteal phase endometrium by tissue microarray assessment. Gynecol Endocrinol, 25, 587-592.
- Smith, G.D., Serafini, P.C., Fioravanti, J., Yadid, I., Coslovsky, M., Hassun, P., Alegretti, J.R. and Motta, E.L. (2010) Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification. Fertil Steril in press